| 无金属卟啉置换辣根过氧化物酶辅基研究 |
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| 引用本文： | 黄琨,王玮,杨卫云,赵莹雪,肖保林,洪军,穆萨维姆瓦赫迪.无金属卟啉置换辣根过氧化物酶辅基研究[J].广州化工,2011,39(11):41-45. |
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| 作者姓名： | 黄琨 王玮 杨卫云 赵莹雪 肖保林 洪军 穆萨维姆瓦赫迪 |
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| 作者单位： | 1. 河南大学生命科学学院,河南开封,475000
2. 德黑兰大学生物化学与生物物理研究所,伊朗德黑兰 |
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| 基金项目： | 河南大学特聘教授科研启动基金和伊朗国家科学基金 |
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| 摘 要： | 不同结构的卟啉插入去辅基辣根过氧化物酶(apo-HRP)所得重构酶具有类过氧化物酶的催化活性.采用紫外分光光度法和荧光光度法对这些重构酶进行研究.结果表明TPyP-apo-HRP比其他卟啉类插入重构蛋白具有的类过氧化物酶催化活性高.
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| 关 键 词： | 卟啉 辣根过氧化物酶 类过氧化物酶 |
Effects of Substituted Metal -free Porphyrins in Apo -horseradish Peroxidase |
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| HUANG Kun,WANG Wei,YANG Wei-yun,ZHAO Ying-xue,XIAO Bao-lin,HONG Jun,MOOSAVI-MOVAHEDI Ali-akbar.Effects of Substituted Metal -free Porphyrins in Apo -horseradish Peroxidase[J].GuangZhou Chemical Industry and Technology,2011,39(11):41-45. |
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| Authors: | HUANG Kun WANG Wei YANG Wei-yun ZHAO Ying-xue XIAO Bao-lin HONG Jun MOOSAVI-MOVAHEDI Ali-akbar |
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| Affiliation: | HUANG Kun1,WANG Wei1,YANG Wei-yun1,ZHAO Ying-xue1,XIAO Bao-lin1,HONG Jun1,MOOSAVI-MOVAHEDI Ali-akbar2(1 College of Life Science,Henan University,Henan Kaifeng 475000,China;2 Institute of Biochemistry and Biophysics,University of Tehran,Tehran,Iran) |
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| Abstract: | The peroxidase-like catalytic activities of several synthetic porphyrins following substitution in apo-horseradish peroxidase(apo-HRP) were studied by UV-vis and fluorescence spectrophotometries in potassium phosphate buffer at pH 7.The insertion of these synthetic porphyrins in apo-HRP was very difficult,due to steric hindrances between coordinated metals in porphyrins and the side-chains of amino acids in native protein.However,because of planar coordination following insertion in an active site of apo-HRP tetrapyridylporphyrin(TPyP),a metal-free porphyrin,adsorbed free metal ions from the environment and showed higher peroxidase-like catalytic activity than other new synthetic porphyrins. |
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| Keywords: | porphyrins HRP peroxidase-like |
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