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| 枯草芽孢杆菌淀粉酶高产菌株的复合诱变研究 | |
| 引用本文： | 马颖辉,王景会,李玉秋,高岩,曹龙奎. 枯草芽孢杆菌淀粉酶高产菌株的复合诱变研究[J]. 中国酿造, 2012, 31(2): 53-56 |
| 作者姓名： | 马颖辉 王景会 李玉秋 高岩 曹龙奎 |
| 作者单位： | 1. 黑龙江八一农垦大学,黑龙江大庆,163319 2. 吉林省农业科学院,吉林长春,130124 3. 吉林农业大学,吉林长春,130175 4. 工业发酵微生物教育部重点实验室,天津科技大学生物工程学院,天津300457 |
| 基金项目： | 吉林省科学技术厅资助项目(20090248) |
| 摘 要： | 该研究以实验室保存的枯草芽孢杆菌为出发菌株，利用紫外和硫酸二乙酯（DES）复合诱变的方法，从大量突变菌株中获取可高效发酵生产淀粉酶的菌株。又通过连续传代实验，确定了该突变菌株突变性状能稳定遗传。结果表明，菌株D-2具有较高的的淀粉酶活力，其淀粉酶活力可达N6．599U／mL，较出发菌株提高到4倍。并在连续培养5代后，仍具有较好的遗传稳定性。该文探讨了一种高效的发酵生产淀粉酶菌株的选育方法，为下一步菌种生长条件优化打下了基础。 |
| 关 键 词： | 复合诱变 枯草芽孢杆菌 淀粉酶 |
Compound mutation of Bacillus subtilis with high-yield of amylase |
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| MA Yinghui , WANG Jinghui , LI Yuqiu , GAO Yan , CAO Longkui. Compound mutation of Bacillus subtilis with high-yield of amylase[J]. China Brewing, 2012, 31(2): 53-56 | |
| Authors: | MA Yinghui WANG Jinghui LI Yuqiu GAO Yan CAO Longkui |
| Affiliation: | 1(1.Heilongjiang Bayi Agricultural University,Heilongjiang 163319,China;2.Jilin Academy of Agriculture Science, Changchun 130124,China;3.Jilin Agricultural University,Changchun 130175,China) |
| Abstract: | The Bacillus strains preserved in our laboratory was used as the original strain to be mutated using UV mutagenesis and diethyl sulfate in order to increase the production of amylase.A mutant strain which was stable in hereditary was obtained.The results indicated that the strain of D-2 had a high production of amylase.The yield of amylase reached 6.599U/ml,which was 4 folds higher than that of the starting strain.A new efficient starch-hydrolysis enzyme strain screening method was developed,which made a good foundation for further medium optimization. |
| Keywords: | compound mutagenesis Bacillus subtilis amylase |
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