中国裸燕麦品种色氨酸含量的荧光检测与评价

本试验旨在对我国主要裸燕麦栽培品种中色氨酸的含量水平进行评价,并分析其与蛋白质含量的关系。利用木瓜酶对新鲜燕麦粉进行水解,在激发波长288 nm和发射波长352 nm处检测水解产物的荧光强度,根据标准曲线对色氨酸含量进行定量。利用全自动蛋白质分析仪检测籽粒中总氮的含量来定量蛋白质,同时对9个裸燕麦栽培品种在4个不同产区种植的蛋白质和色氨酸含量分别进行检测并作显著性差异和相关性分析。结果表明,中国裸燕麦品种平均的色氨酸含量为2.39 mg/g,大于国外报道的皮燕麦品种平均色氨酸含量1.70 mg/g。品种和地区以及两者互作引起的燕麦色氨酸含量的差异显著,且燕麦色氨酸和蛋白质含量之间具有一定的相关关系,相关系数为0.353。

Assessment and determination of tryptophan in Chinese oat grain using fluorescence spectroscopy

Cereal are important sources of protein for human nutrition but have low quality due to limitations in the amounted of essential amino acid, lysine, and tryptophan. Oat Oats (Avena sativa L.) are distinct among cereals due to their considerably higher protein concentration and balanced amino acid. The tryptophan content is also higher than the other cereals. The determination of tryptophan content is a method to assese the quality of grain protein. The present study aims to assess the total protein and the tryptophan content of chinese naked oats varities. A rapid method of determination of tryptophan content in oats grain using fluorescence spectroscopy was introduced. Oat flour were hydrolysed with papain, the fluorescence of hydralate were determined at exciting 288nm, and emission 352nm, tryptophan content were calculated by standard curve. Meanwhile, the tryptophan and protein content were determined for 9 different varieties grown in 4 different location in China, moreover, the variation and correlation of tryptophan were analyzed as well. The result showed that there was significant diference between locations, varieties, and interaction. A linear correlation between protein and tryptophan cotent was also found in the present study. Comparing to the tryptophan content reported in hulled oats before which was 1.7mg/g, chinese naked oat has higher value, the average was 2.39mg/g.