2种检测变形杆菌PCR方法的比较研究

选取atpD基因作为靶序列设计了2对引物,分别采用不同的PCR扩增条件,对3株变形杆菌属标准菌株,67株样品分离株及13株非变形杆菌属菌株进行扩增实验,结果2种PCR方法对3株标准菌株和66株样品分离株分别扩增出348 bp和596 bp的特异性片断,实验结果呈阴性的1株分离株经全自动微生物鉴定仪鉴定为阴沟肠杆菌。13株非变形杆菌属菌株均未有特异性条带产生。2种PCR方法检测变形杆菌,均具有快速、准确、灵敏、特异的优点。建立的引物2的PCR方法在检测时间、特异性、灵敏度方面比引物1的PCR方法更具优势。

Comparison of Two Detection Method in Proteus by Polymerase Chain Reaction

Two pairs of primers were designed by taking the aptD gene as objective sequences to amplify the genes of 3 references Proteus,67 isolated Proteus and 13 non-Proteus strains under two different PCR conditions.Expected 348 bp and 596 bp fragments were obtained for 3 references Proteus and 66 isolated Proteus.The negative 1 strain was detected as Enterobacter cloacae by Vitek Auto Microbe System.No spe- cific fragments were detected in the 13 strains of the non—Proteus bacteria.The two PCR methods were rap- id,concise,sensitive and specific to the detection of Proteus strains.However,primer 1 method showed more advantages in time length,specificity and sensitivity to Proteus detection compared to the primer 2.