| 一株产多糖芽孢杆菌的筛选与鉴定 |
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| 引用本文： | 张彩,李彦岩,范熠,刘丽莎,李平兰. 一株产多糖芽孢杆菌的筛选与鉴定[J]. 中国酿造, 2012, 0(10): 82-85 |
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| 作者姓名： | 张彩 李彦岩 范熠 刘丽莎 李平兰 |
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| 作者单位： | 中国农业大学食品科学与营养工程学院,北京100083 |
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| 基金项目： | 国家大学生创新性实验计划项目（201210019088） |
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| 摘 要： | 从来源于面酱、燕麦、康乃馨等食品、天然物质的132株芽孢杆菌中，以胞外多糖产量为指标，筛选出一株来源于康乃馨的多糖高产菌株LPL061。采用LB培养基，发酵温度37℃，转速150r／min，发酵时间24h，多糖产量为1．751g／L。通过形态学、牛珲牛化鉴定、16SrDNA序列同源性分析和特异性基因序yrJPCR，鉴定目标菌株为解淀粉芽孢杆菌（Bacillus amyloliquefaciens LPL061）。
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| 关 键 词： | 解淀粉芽孢杆菌 胞外多糖 鉴定 |
Screening and identification of an exopolysaccharide-producing Bacillus sp. |
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| ZHANG Cai,LI Yanyan,FAN Yi,LIU Lisha,LI Pinglan. Screening and identification of an exopolysaccharide-producing Bacillus sp.[J]. China Brewing, 2012, 0(10): 82-85 |
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| Authors: | ZHANG Cai LI Yanyan FAN Yi LIU Lisha LI Pinglan |
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| Affiliation: | (College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China) |
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| Abstract: | An exopolysaccharide-producing strain Bacillus LPL061 from carnation was screened from the 132 Bacillus separated from 30 kinds of food and natural materials. The exopolysaccharide productivity of LPL061 cultured in LB fermentation medium, the initial pH value, temperature and incubation time of which were pH 7.0, 37~C and 24h, is 1.75 lg/L. Based on morphological characteristics, physiological and biochemical identification, 16S rDNA sequence and specific genes analysis, LPL061 was identified as Bacillus amyloliquefaciens. |
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| Keywords: | Bacillus amyloliquefaciens exopolysaccharide identification |
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