高效液相色谱内标法测定香精香料中的水杨酸

建立了香精香料中水杨酸的液相色谱检测方法。该方法以甲醇提取样品中的水杨酸,3,4-二羟基苯甲酸为内标;梯度洗脱,流动相分别为乙腈和50mmol·L-1磷酸溶液,DAD检测器检测,内标法定量。结果表明,水杨酸在0.05～1.00μg·mL-1范围内线性关系良好,拟合度为0.9999,回收率为97.53%～98.13%,相对标准偏差为1.08%～1.37%。

Determination of salicylic acid in flavor and essence by HPLC internal standard method

The determination method of salicylic acid in flavor and essence was established by high performance liquid chromatography.Samples were extracted with methanol.The mobile phase was acetonitrile and 50mmol·L^-1 phosphoric acid,gradient elution,DAD detection,quantified by internal standard method.The results show that the calibration curve of salicylic acid at the concentration range of 0.05～1.00μg·mL-1 was linear（R2= 0.9999）.The recoveries of the salicylic acid ranged from 97.53% to 98.13% with relative standard deviations between 1.08%～1.37%.