| 表达人粒细胞集落刺激因子大肠杆菌的优化 |
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| 引用本文： | 马骊,方向东.表达人粒细胞集落刺激因子大肠杆菌的优化[J].粉末涂料与涂装,1999,12(2):75-77. |
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| 作者姓名： | 马骊 方向东 |
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| 作者单位： | 第一军医大学分子免疫学研究所!广州510515 |
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| 摘 要： | 通过对hG CSF重组基因表达效率和工程菌遗传稳定性的比较 ,建立的一种全新的双质粒双重诱导的原核高效表达体系。构建了pGP1 2 pJGW1 hGCSF DH5α工程菌 ,经 42℃热诱导 ,hG CSF的表达率达 2 7%以上 ,经典质粒遗传稳定性实验 (140代 )证实 ,具有良好的遗传稳定性。
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| 关 键 词： | 重组人粒细胞集落刺激因子 原核表达 稳定性 |
Improvement of Expression System of Human Granulocyte Colony Stimulating Facter in E.coli |
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| Ma Li,Fang Xiangdong,Gao Jimin et al.Improvement of Expression System of Human Granulocyte Colony Stimulating Facter in E.coli[J].Chinese Journal of Biologicals,1999,12(2):75-77. |
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| Authors: | Ma Li Fang Xiangdong Gao Jimin |
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| Abstract: | A novel double induced expression system containing plasmids pJGW1 hGCSF and pGP1 2 was constructed on the basis of study on the expression efficiency of recombinant hG CSF gene and the genetic stability of enginerring E.coli.After the recombinant bacterial strain,pGP1 2 pJGW1 hGCSF/DH5α,was induced at 42℃,27% of hG CSF was expressed.The recombinant bacterial strain had been undergone 140 passages,the result showed the good genetic stability of it. |
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| Keywords: | rhG CSF Expression Stability |
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