应用不同生产工艺制备甲肝(L-A-1)减毒活疫苗的质量比较

采用细胞消化传代同时加疫苗病毒感染细胞的方法 (简称同时感染法 )与细胞长成单层后再加病毒感染细胞的方法 (简称单层感染法 )所制备的疫苗进行质量比较 ,结果同时感染法的生产周期明显缩短 ,病毒达增殖高峰期的细胞仍能保持良好的形态 ,疫苗病毒滴度明显提高。表明该工艺更适合于规模化、工业化生产

Comparison of Qualities of Live Attenuated Hepatitis A Vaccine (Strain L-A -1) Prepared by Different Processes

The qualities of live attenuated hepatitis A(HA) vaccine (strain L A 1) prepared by 2 different processes were compared. In the lst process, the cells were infected with HA at the same time of passage; however, in the 2nd process, they were infected after monolayer was formed. The result showed that, by the 1st process, the production cycle of vaccine was significantly shortened, the virus titer of it was significantly improved, and, even at the peak time of virus proliferation, no significant cytomrphological change was observed. It indicated that the 1st process was more suitable for the large scale production.