Entrapment of urease in poly(1‐vinyl imidazole)/poly(2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid) network |
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| Authors: | Mehmet Şenel Emre Çevik M. Fatih Abasıyanık Ayhan Bozkurt |
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| Affiliation: | 1. Department of Chemistry, Faculty of Arts and Sciences, Fatih University, B.Cekmece, Istanbul 34500, Turkey;2. Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500, Turkey |
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| Abstract: | In this article, urease was immobilized in a conducting network via complexation of poly(1‐vinyl imidazole) (PVI) with poly(2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid) (PAMPS). The preparation method for the polymer network was adjusted by using Fourier transform infrared (FTIR) spectroscopy. A scanning electron microscope (SEM) study revealed that enzyme immobilization had a strong effect on film morphology. The proton conductivity of the PVI/PAMPS network was measured via impedance spectroscopy, under humidified conditions. The basic characteristics (Michealis‐Menten constants, pHopt, pHstability, Topt, Tstability, reusability, and storage stability) of the immobilized urease were determined. The obtained results showed that the PAA/PVI polymer network was suitable for enzyme immobilization. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011 |
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| Keywords: | urease immobilization proton conductivity polymernNetwork |
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