| RP-HPLC法测定复方大青叶颗粒中靛玉红含量 |
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| 引用本文： | 刘迎春,张莹,段丽颖,王国清,王金萃,唐旭. RP-HPLC法测定复方大青叶颗粒中靛玉红含量[J]. 化学世界, 2009, 50(12) |
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| 作者姓名： | 刘迎春 张莹 段丽颖 王国清 王金萃 唐旭 |
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| 作者单位： | 沈阳药科大学制药工程学院,辽宁,沈阳,110016;沈阳药科大学制药工程学院,辽宁,沈阳,110016;沈阳药科大学制药工程学院,辽宁,沈阳,110016;沈阳药科大学制药工程学院,辽宁,沈阳,110016;沈阳药科大学制药工程学院,辽宁,沈阳,110016;沈阳药科大学制药工程学院,辽宁,沈阳,110016 |
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| 摘 要： | 建立了一种RP-HPLC法测定复方大青叶颗粒中靛玉红的含量。样品用Kromasil C18(200 mm×4.6 mm,5μm)柱分离,检测波长为289 nm,流动相为甲醇-水(体积比为75∶25),流速为1.0 mL/min。靛玉红在1.25~19.97 mg/L范围内与峰面积呈良好线性关系,r=0.9999,平均回收率为102.0%(RSD=1.5%,n=9)。该法准确、快速、简单,适合复方大青叶颗粒中靛玉红的含量测定。
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| 关 键 词： | 复方大青叶颗粒 靛玉红 RP-HPLC法 含量测定 |
Content Determination of Indirubin in Compound Daqingye Grains by RP-HPLC |
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| LIU Ying-chun,ZHANG Ying,DUAN Li-ying,WANG Guo-qing,WANG Jin-cui,TONG Xu. Content Determination of Indirubin in Compound Daqingye Grains by RP-HPLC[J]. Chemical World, 2009, 50(12) |
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| Authors: | LIU Ying-chun ZHANG Ying DUAN Li-ying WANG Guo-qing WANG Jin-cui TONG Xu |
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| Abstract: | A RP-HPLC method for content determination of indirubin in compound Daqingye grains was established.The sample was separated by using column C_(18)(200 mm×4.6 mm,5μm),and the mobile flow rate was 1.0 mL/min.The linear range of indirubin was 1.25～19.97 mg/L(r=0.9999.).The mean recovery was 102.0%(RSD=1.5%,n=9).The methods is accurate,rapid and simple,and it is suitable for content determination of indirubin in Compound Daqingye grains. |
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| Keywords: | compound Daqingye grains indirubin RP-HPLC content determination |
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