| 人血清IgG的188Re标记条件初探 |
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| 引用本文： | 丁自更,于俊峰,尹端,胡伟清,汪勇先,王根凤,张茹萍,来文,谢弘.人血清IgG的188Re标记条件初探[J].核技术,2000,23(11):762-766. |
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| 作者姓名： | 丁自更 于俊峰 尹端 胡伟清 汪勇先 王根凤 张茹萍 来文 谢弘 |
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| 作者单位： | 1. 中国科学院上海原子核研究所 上海201800
2. 中国科学院上海细胞生物研究所 上海200031 |
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| 摘 要： | 以含葡萄糖酸钠配体的SnCl2为还原液，还原Na188ReO4后，标记人血清IgG，在pH=3的条件下，1g/L的蛋白质用高于5000倍摩尔过量的抗坏血酸(AA)还原2h后， 与2.6×10-28.0×10-2mol/L的SnCl2-葡萄糖酸钠溶液及188ReO4-反应3h，可以得到90%的标记率，在该标记条件下，对影响标记率的因素作了初步的研究和探讨。
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| 关 键 词： | 标记率 人血清白蛋白 铼^188标记 放射性核素 |
| 修稿时间： | 1999年3月5日 |
A preliminary study on the labelling of immunoglobulin with 188Re |
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| DING Zigeng,YU Junfeng,YIN Duanzhi,HU Weiqing,Wang Yongxian,WANG Genfeng,ZHANG Ruping,LAI Wen,XIE Hong.A preliminary study on the labelling of immunoglobulin with 188Re[J].Nuclear Techniques,2000,23(11):762-766. |
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| Authors: | DING Zigeng YU Junfeng YIN Duanzhi HU Weiqing Wang Yongxian WANG Genfeng ZHANG Ruping LAI Wen XIE Hong |
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| Abstract: | SnCl2 reduction solution together with sodium gluconate was chosen to reduce Na188ReO4- and label human IgG. The labellin
g conditions were optimized. It was found that the labelling efficiency could reach 90% under the following condition: 1g/L protein with 5000 mole r
atio AA in 2h reducing time at pH=3, in mingled with 2.6×10-28.0×10-2mol/L SnCl2 in sodium gluconate and 188ReO4- at ambient temperature. The fa
ctors effecting labelling efficiency were studied and discussed. |
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| Keywords: | Labelling efficiency 188Re Human immunoglobulin |
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