Rapid Enzymatic Assay for Ascorbic Acid in Various Foods Using Peroxidase |
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| Authors: | F. TSUMURA Y. OHSAKO Y. HARAGUCHI H. KUMAGAI H. SAKURAI K. ISHII |
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| Affiliation: | The authors are with the Dept. of Agricultural Chemistry, College of Agriculture &Veterinary Medicine, Nihon Univ., 3–34–1, Shimouma, Setagaya-ku, Tokyo 154, Japan. Address inquiries to Dr. H. Kumagai. |
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| Abstract: | ![]()
Based on direct spectrophotometry and the oxidation of ascorbic acid by guaiacol peroxidase, this method was simple, rapid and highly specific for ascorbic acid. No interference was seen for any food sample tested, including vegetables, fruits, potatoes, beans, chicken liver, processed food, tea and seaweed. This method was applicable to colored and/or sugar-rich samples, and more precise than officially adopted chemical methods. |
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| Keywords: | guaiacol peroxidase ascorbic acid spectrophotometry vitamin C |
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