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| 大团囊虫草菌胞内多糖发酵优化和抗氧化活性研究(英文) | |
| 引用本文： | 许勤勤,刘振华,孙一晟,丁忠杰,吕龙贤,李永泉. 大团囊虫草菌胞内多糖发酵优化和抗氧化活性研究(英文)[J]. 中国化学工程学报, 2012, 20(2): 294-301. DOI: 10.1016/S1004-9541(12)60391-7 |
| 作者姓名： | 许勤勤 刘振华 孙一晟 丁忠杰 吕龙贤 李永泉 |
| 作者单位： | College of Life Sciences, Zhejiang University, Hangzhou 310058, China |
| 基金项目： | Supported by the National High Technology Research and Development Program of China (2007AA021506);the Natural Science Foundation of Zhejiang Province (R207609);the Research Project of Science and Technology of Zhejiang Province,China (2005C23027) |
| 摘 要： | Cordyceps ophioglossoides is a valuable traditional medicinal material.We have found that intracellular polysaccharide(IPS) is the major biologically active ingredient in Cordyceps ophioglossoides.This study is the first time to optimize the yield of IPS from Cordyceps ophioglossoides.The optimal medium for IPS production consists of glucose 54.50 g·L·1,yeast powder 25.50 g·L·1,NaH2PO4 0.4 g·L·1 and K2HPO4 0.4 g·L·1.The suggested culture conditions are 24 ℃,initial pH 4.5 with a rotary speed of 120 r·min·1 for 168 h.The yield of IPS is 737.93 mg·L·1,which is 50% higher than the yield under the conditions prior to optimization.The anti-oxidative activities of IPS in Cordyceps ophioglossoides L2 are also characterized using various in vitro assay.The anti-oxidative activity may explain the reason why IPS from Cordyceps ophioglossoides can be used to fight against neurodegenerative dis-eases and menopausal symptoms. |
| 关 键 词： | antioxidant activity Cordyceps ophioglossoides L2 intracellular polysaccharide submerged culture optimization |
| 收稿时间： | 2011-05-11 |
Optimization for Production of Intracellular Polysaccharide from Cordyceps ophioglossoides L2 in Submerged Culture and Its Antioxidant Activities in vitro |
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| XU Qinqin,LIU Zhenhua,SUN Yisheng,DING Zhongjie,Lü Longxian and LI Yongquan. Optimization for Production of Intracellular Polysaccharide from Cordyceps ophioglossoides L2 in Submerged Culture and Its Antioxidant Activities in vitro[J]. Chinese Journal of Chemical Engineering, 2012, 20(2): 294-301. DOI: 10.1016/S1004-9541(12)60391-7 | |
| Authors: | XU Qinqin LIU Zhenhua SUN Yisheng DING Zhongjie Lü Longxian LI Yongquan |
| Affiliation: | College of Life Sciences, Zhejiang University, Hangzhou 310058, China |
| Abstract: | Cordyceps ophioglossoides is a valuable traditional medicinal material.We have found that intracellular polysaccharide(IPS) is the major biologically active ingredient in Cordyceps ophioglossoides.This study is the first time to optimize the yield of IPS from Cordyceps ophioglossoides.The optimal medium for IPS production consists of glucose 54.50 g·L·1,yeast powder 25.50 g·L·1,NaH2PO4 0.4 g·L·1 and K2HPO4 0.4 g·L·1.The suggested culture conditions are 24 ℃,initial pH 4.5 with a rotary speed of 120 r·min·1 for 168 h.The yield of IPS is 737.93 mg·L·1,which is 50% higher than the yield under the conditions prior to optimization.The anti-oxidative activities of IPS in Cordyceps ophioglossoides L2 are also characterized using various in vitro assay.The anti-oxidative activity may explain the reason why IPS from Cordyceps ophioglossoides can be used to fight against neurodegenerative dis-eases and menopausal symptoms. |
| Keywords: | antioxidant activity Cordyceps ophioglossoides L2 intracellular polysaccharide submerged culture optimization |
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