益生菌对大鼠肾功能缺失性肠屏障损伤的修复作用

肾功能缺失对机体的危害之一是肠屏障损伤。本研究对大鼠进行5/6肾切除手术,灌胃益生菌,研究益生菌对大鼠肾病所致肠屏障损伤及相关炎症反应的修复作用。结果表明:灌胃混合益生菌6×1010CFU/d 10周后能够显著改善受损伤肠屏障。血清检测发现,灌注益生菌后脂多糖含量从0.19 EU/mL降到0.10 EU/mL(P<0.05),说明益生菌成功阻止肠道微生物的代谢物透过肠道屏障进入机体;肠上皮隐窝深度和黏膜厚度显著增加(P<0.05),杯状细胞数量显著上升(P<0.05),紧密连接蛋白ZO-1和claudin-1的分泌显著增加(P<0.05),受损的肠道屏障得到修复。灌胃益生菌后,血清中C-反应蛋白和TNF-α等促炎因子的含量显著降低(P<0.05),IL-10和IL-13等抗炎因子的含量显著提高(P<0.05),表明益生菌修复后的肠道屏障调节了促炎因子和抗炎因子的平衡,使机体的系统炎症得到有效控制。

Effects of Probiotics on Impaired Intestinal Barrier Induced by Renal Dysfunction in Rats

Impaired intestinal barrier commonly occurs during the renal dysfunction. In this study, we assessed the effect of probiotics on impaired intestinal barrier and related inflammation in 5/6 nephrectomy rat model(CKD rats). The results showed that CKD rats impaired intestinal barrier was significantly imporved by gavage administration of 6 ×1010 CFU/d of probiotics mixture for ten weeks. treated by probiotics mixture product had remarkably lower lipopolysaccharide(LPS) in serum(from 0.19 EU/mL to 0.10 EU/mL, P<0.05). This indicated that probiotics mixture reduced intestinal permeability. Taking probiotics mixture significantly increased crypt depth, thickness of mucosa and the number of goblet cells(P <0.05) in intestinal epithelium. Administration of probiotics mixture also increase expression of of ZO-1 and claudin-1(P<0.05) indicating the improvement of tight junction. Furthermore, Taking probiotics mixture significantly decreased the level of C-reaction protein and TNF-α (P<0.05), and increased the level of IL-10 and IL-13(P<0.05) in serum indicating balanced proinflammatory and anti-inflammatory reponses of host.