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Screening of supports for the immobilization of pectinmethylesterase from acerola (Malpighia glabra L)
Authors:Sandra Aparecida de Assis  Pedro Fernandes  Bruno Sommer Ferreira  Joaquim MS Cabral  Olga Maria Mascarenhas Faria Oliveira
Abstract:A partially purified extract of pectinmethylesterase (PME) from acerola fruit was immobilized on various supports: glass, celite, chrysotile, agarose, concanavalin A Sepharose 4B, egg shell, polyacrylamide and gelatin. In addition, reticulation with glutaraldehyde was assessed, as well as the use of gelatin in the presence of celite, glass and silica. The highest immobilization yields were obtained when the pectinmethylesterase was immobilized in concanavalin A Sepharose 4B (81.7%) and in gelatin‐water (78.0%). Copyright © 2004 Society of Chemical Industry
Keywords:acerola  pectinmethylesterase  immobilization  kinetics characterization
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