Specific determination of malonaldehyde by N-methyl-2-phenylindole or thiobarbituric acid |
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Authors: | Tadamichi Inoue Ken Ando Kiyomi Kikugawa |
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Affiliation: | (1) School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, 192-0392 Tokyo, Japan |
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Abstract: | Reactivity of a commercially available test kit (LPO-586), based on N-methyl-2-phenylindole, toward aldehydes was characterized and compared with that of thiobarbituric acid (TBA). In hydrochloric
acid, LPO-586 produced a violet pigment with malonaldehyde (MA) but not with other tested aldehydes. In methane sulfonic acid,
LPO-586 produced the violet pigment with MA and 4-hydroxynonenal (HNE), but not with other tested aldehydes. Pigment formation
with MA was not inhibited by other aldehydes, but that with HNE was inhibited by alka-2,4-dienals. TBA produced a red pigment
with MA but not with other tested aldehydes in hydrochloric acid or in acetate with ethylenediaminetetraacetic acid (EDTA).
Both the LPO-586 test in hydrochloric acid and the TBA test in hydrochloric acid or in acetate with EDTA can be used for specific
measurement of MA in oxidized lipid samples. |
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Keywords: | Alka-2 4-dienal 4-hydroxyalkenal lipid oxidation malonaldehyde N-methyl-2-phenylindole thiobarbituric acid |
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