| 海洋纤溶酶高产菌株的选育及产酶条件优化 |
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| 引用本文： | 黎艳,张兆峰,田苗,张云开,陈桂光,梁智群. 海洋纤溶酶高产菌株的选育及产酶条件优化[J]. 中国酿造, 2012, 31(2): 33-37 |
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| 作者姓名： | 黎艳 张兆峰 田苗 张云开 陈桂光 梁智群 |
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| 作者单位： | 广西大学 生命科学与技术学院,广西南宁,530004 |
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| 基金项目： | 国家自然科学基金资助项目(21062001) |
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| 摘 要： | 以海洋枯草芽孢杆菌FA-7为出发菌株，经紫外诱变获得一株遗传稳定性良好的高产纤溶酶菌株Y-22，并对该菌株的发酵培养基和发酵条件进行优化，确定菌株Y-22的最佳产酶条件为可溶性淀粉4％，黄豆粕粉2．5％，CaCl20．025％，MgSO4·7H：00．35％，吐温-80O．15％；接种量4％，装液量50mt：250mL，初始pH值为5．5，发酵温度30℃，180r／min振荡培养96h。在此条件下，菌株Y-22的发酵液粗酶酶活为（910±11．3）1U／mL，是出发菌株的3．05倍。
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| 关 键 词： | 海洋枯草芽孢杆菌 纤溶酶：诱变育种 优化 |
Mutagenesis breeding of a high fibrinolytic enzyme strain and the optimization of its fermentation conditions |
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| LI Yan , ZHANG Zhaofeng , TIAN Miao , ZHANG Yunkai , CHENG Guiguang , LIANG Zhiqun. Mutagenesis breeding of a high fibrinolytic enzyme strain and the optimization of its fermentation conditions[J]. China Brewing, 2012, 31(2): 33-37 |
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| Authors: | LI Yan ZHANG Zhaofeng TIAN Miao ZHANG Yunkai CHENG Guiguang LIANG Zhiqun |
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| Affiliation: | (College of Life Science and Technology,Guangxi University,Nanning 530004,China) |
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| Abstract: | In this work,a marine Bacillus subtilis which produces fibrinolytic enzyme was mutagenesis by UV.A mutant(Y-22) contained high fibrinolytic activity was obtained.It was stable after several generations.The optimal medium and cultured conditions were as follows: soluble starch 4%,soybean meal 2.5%,CaCl2 0.025%,MgSO4·7H2O 0.35%,Tween-80 0.15%,inoculum 4%,liquid volume 50ml/250ml(flask),the initial pH value 5.5,temperature 30℃ and the rotation speed 180r/min for 96h.Under these conditions,the activity of fibrinolytic enzyme reached(910±11.3)IU/ml,which was 3.05 folds as high as that of the initial strain. |
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| Keywords: | marine Bacillus subtilis fibrinolytic enzyme mutagenesis breeding optimization |
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