高效液相色谱- 电感耦合等离子体质谱联用测定婴幼儿辅食中的无机砷

目的：拟建立婴幼儿辅助食品中无机砷含量的测定方法。方法：以0.15mmol/L硝酸溶液为提取剂，90℃热浸提取婴幼儿辅助食品(米粉类及罐头类)中无机砷，采用Hamilton PRP-X100(250mm×4.1mm，10μm)色谱柱分离，建立高效液相色谱-电感耦合等离子体质谱(high performance liquid chromatography-inductively coupled plasma mass spectrometry，HPLC-ICP/MS)联用技术测定其含量的方法。结果表明：As(Ⅲ)与As(Ⅴ)的线性范围为0.5～400μg/L，线性相关系数(r)优于0.9990，检出限分别为0.06μg/L和0.08μg/L，无机砷的加标回收率在80.5%～128.5%之间，相对标准偏差(RSD)＜5%。结论：方法简便、准确、灵敏，适用于婴幼儿辅助食品中无机砷含量的测定。

Determination of Inorganic Arsenic in Complementary Foods for Infants and Young Children by HPLC-ICP/MS

Objectives: To establish a high performance liquid chromatography-inductively coupled plasma mass spectrometry method for the determination of inorganic arsenic in complementary food for infants and young children. Methods: Sample preparation was achieved by extraction with 0.15 mmol/L nitric acid at 90 ℃. Chromatographic separation was performed on a Hamilton PRP-X100 column (250 mm × 4.1 mm, 10 μm). Results: The linear ranges for As (Ⅲ) and As (Ⅴ) were both between 0.5 μg/L and 400 μg/L with a correlation coefficient of more than 0.9990. The detection limits for As (Ⅲ) and As (Ⅴ) were between 0.06 μg/L and 0.08 μg/L, and the spike recoveries were in the range of 80.5%－128.5% with a relative standard deviation (RSD) of less than 5%. Conclusions: This method was simple, sensitive and accurate, and could be used to determine the inorganic arsenic levels in complementary foods for infants young children.