Astaxanthin Extraction From Shrimp Waste by Lactic Fermentation and Enzymatic Hydrolysis of the Carotenoprotein Complex |
| |
| Authors: | R Armenta-López I L Guerrero S Huerta |
| |
| Affiliation: | The authors are with the Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa. Apartado Postal 55-535. C.P. 09340, México, D.F. Mexico. Direct inquiries to author Guerrero L. (). |
| |
| Abstract: | ABSTRACT: Natural carotenoids are an alternative to synthetic orange-red pigments. They are present in crustaceans as a protein-pigment complex. In order to extract this highly unstable pigment, crustacean waste must be stabilized; lactic fermentation is a simple and environmentally friendly method to achieve this goal. Shrimp wastes were inoculated with Lactobacillus bacterial cultures. Carotenoids were then extracted with an organic solvent system. Protein-pigment splitting was carried out using a mixture of 4 commercial enzymes; and the protein was separated from the pigment by ultrafiltration. Electrophoretograms showed that the pigment was attached to a 265-kDa protein. Splitting the protein-pigment complex allows studies on pigment absorption, stability and application. |
| |
| Keywords: | astaxanthin carotenoids crustacean waste lactic fermentation enzymatic hydrolysis |
|
