椰子油和大豆油的乙氧基化物对小鼠肝脏的脂质过氧化效应研究

为探讨椰子油乙氧基化物-36EO(COE-36)和大豆油乙氧基化物-42EO(SOE-42)对小鼠肝脏的脂质过氧化作用,采用腹腔注射技术,测定COE-36处理组、SOE-42处理组、氯化镉阳性对照组(小鼠单位体质量的氯化镉用量为2.5μg.g-1)和生理盐水阴性对照组小鼠肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和丙二醛(MDA)生成量。结果表明,氯化镉显著抑制了小鼠肝脏SOD和CAT活性,增加了MDA生成量;在所测3个指标中,COE-36和SOE-42的3个用量组(以小鼠单位体质量的用药量计)与生理盐水阴性对照组相比均无显著性差异;与氯化镉阳性对照组相比,不同用量COE-36作用下SOD和CAT活性变化、不同用量SOE-42作用下CAT活性变化以及低用量(3 mg.g-1)COE-36或SOE-42作用下MDA生成量变化均有显著性差异,说明在所试用量(3～5 mg.g-1)下,这2种表面活性剂不会引起小鼠肝细胞的脂质过氧化。

Effect of coconut oil and soybean oil ethoxylates on peroxidation of liver tissue lipid of mice

In order to investigate effects of coconut oil ethoxylates(COE-36) and soybean oil ethoxylates(SOE-42) on peroxidation of liver tissue lipid of mice,activity of superoxide dismutase(SOD) and catalase(CAT) as well as amount of malonaldehyde(MDA) formed in liver of mice of COE-36 treatment group,SOE-42 treatment group,cadmium chloride treatment(positive control test) group(2.5 μg·g-1 based upon mass of mice) and physiological saline treatment(negative control test) group were tested and measured respectively via intraperitoneal injection technology.The results showed that cadmium chloride significantly inhibits the three indices(activity of SOD and CAT and amount of MDA formed in mice liver) as compared with that of saline treatment group.The changes of the three indices in mice liver of COE-36 treatment groups as well as SOE-42 treatment groups with three different dosage levels are not significant as compared with that of saline treatment group.The changes of SOD and CAT activity in COE-36 treatment groups with three different dosage levels,the change of CAT activity in SOE-42 treatment groups with three different dosage levels and the change of MDA formed in mice liver of COE-36 and SOE-42 treatment groups with low dosage(3 mg·g-1 COE-36 and SOE-42 respectively) are significant as compared with that of cadmium chloride treatment group.The observations suggested that COE-36 or SOE-42 in the tested dosages(3~5 mg·g-1) would not induce peroxidation in lipid of mice liver tissues.