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| 转基因玉米的七重PCR鉴定方法 | |
| 引用本文： | 陈贞,芦春斌,杨梦婕,马骏,白卫斌,吴希阳.转基因玉米的七重PCR鉴定方法[J].食品与发酵工业,2011,37(6). |
| 作者姓名： | 陈贞 芦春斌 杨梦婕 马骏 白卫斌 吴希阳 |
| 作者单位： | 1. 暨南大学理工学院食品科学与工程系,广东广州,510632 2. 暨南大学生殖免疫研究所,广东广州,510632 3. 广东出入境检验检疫局检验检疫技术中心,广东广州,510623 |
| 基金项目： | 农业部转基因重大专项“转基因生物检测和监测新技术”(2008ZX08012-005) |
| 摘 要： | 以玉米内源基因IVR、外源抗除草剂基因(BAR、PAT)、抗虫基因Cry1Ab、筛选基因NPTII、CaMV35S启动子和NOS终止子为检测的目的片段,分别设计了7对引物,通过研究较佳引物终浓度配比和退火温度,建立了玉米转基因成分七重PCR检测体系。结果表明,建立的七重PCR体系用于同时检测内源基因和转基因成分是可行的,检测方法效率高、稳定性好。 |
| 关 键 词： | 玉米 转基因成分 七重PCR检测 |
Seven-Plex PCR Detection Method for Genetically Modified Maize |
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| Chen Zhen,Lu Chun-bin,Yang Meng-jie,Ma Jun,Bai Wei-bin,Wu Xi-yang.Seven-Plex PCR Detection Method for Genetically Modified Maize[J].Food and Fermentation Industries,2011,37(6). | |
| Authors: | Chen Zhen Lu Chun-bin Yang Meng-jie Ma Jun Bai Wei-bin Wu Xi-yang |
| Affiliation: | Chen Zhen1,Lu Chun-bin2,Yang Meng-jie2,Ma Jun3,Bai Wei-bin1,Wu Xi-yang1 1(Department of Food Science and Engineering,Jinan University,Guangzhou 510632,China) 2(Institute of Reproductive I mmol/Lunology,China) 3(Guangdong Entry-Exit Inspection and Quarantine Bureau Inspection and Quarantine Technology Center,Guangzhou 510623,China) |
| Abstract: | To establish Seven-plex PCR detection system for detecting transgenic components in genetically modified Maize,endogenous gene IVR,exogenous herbicide gene BAR and PAT,exogenous insect resistant gene Cry1Ab,screening gene NPTII,CaMV35S promoter and Nos terminator were chosen for the targeted fragment.7 pairs of primers are designed and the PCR conditions for the multiplex PCR analysis were optimized according to the primer concentration and annealing temperature.The results show that Seven-Plex PCR system c... |
| Keywords: | maize genetically modified components Seven-Plex PCR detection |
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