| 化学发光酶免疫法检测玉米中玉米赤霉烯酮 |
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| 引用本文： | 卢江,许杨,楚金申,谢琳. 化学发光酶免疫法检测玉米中玉米赤霉烯酮[J]. 食品与发酵工业, 2011, 37(5) |
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| 作者姓名： | 卢江 许杨 楚金申 谢琳 |
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| 作者单位： | 南昌大学食品科学与技术国家重点实验室,中德联合研究院,江西南昌330047 |
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| 基金项目： | 国家“863”计划项目(2007AA10Z427) |
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| 摘 要： | 应用抗玉米赤霉烯酮单克隆抗体,以辣根过氧化物酶催化鲁米诺-过氧化氢化学发光体系,建立了一种简便、快速、高特异性的间接竞争化学发光酶免疫法用于检测玉米中的玉米赤霉烯酮。所建立的方法分析灵敏度为0.09 ng/mL,批内变异系数小于9.1﹪,批间变异系数小于14.7﹪,样品的加标回收率为79.6﹪～97.6﹪,所建立的标准曲线相关系数为0.9884,检测线性范围为0.104～1.69 ng/mL,检测时间为60 min。
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| 关 键 词： | 玉米赤霉烯酮(ZEN) 间接竞争化学发光酶免疫法(CLEIA) 检测 |
Chemiluminescence Enzyme Immunoassay for the Determination of Zearalenone in Corn |
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| Lu Jiang,Xu Yang,Chu Jin-shen,Xie Lin. Chemiluminescence Enzyme Immunoassay for the Determination of Zearalenone in Corn[J]. Food and Fermentation Industries, 2011, 37(5) |
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| Authors: | Lu Jiang Xu Yang Chu Jin-shen Xie Lin |
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| Affiliation: | Lu Jiang,Xu Yang,Chu Jin-shen,Xie Lin(Sino-Germany Joint Research Institute,The State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China) |
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| Abstract: | A sensitive indirect competitive chemiluminescence enzyme immunoassay(CLEIA) for zearalenone in corn has been developed.The optimized CLEIA gave a limit detection of 0.09 ng/mL and a detection range of 0.104-1.69 ng/mL,with an IC50 of 0.438 1 ng/mL and it was about 3 times more sensitive than that of colorimetric ELISA.It has been validated on cereals samples in terms of precision(intra-and interassay coefficient variations of less than 9.1﹪ and 14.7﹪,respectively) and accuracy(mean recovery 79.6﹪~97.6﹪).These assay performances indicated that CLEIA was capable of being applied for the specific detection and routine monitoring of ZEN in corn samples. |
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| Keywords: | zearalenone(ZEN) chemiluminescence enzyme immunoassay(CLEIA) determination |
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