| 外电场对苯丙氨酸在双水相体系中分配的影响 |
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| 引用本文： | 刘蕾,骆广生,吕阳成.外电场对苯丙氨酸在双水相体系中分配的影响[J].过程工程学报,2003,3(4):317-322. |
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| 作者姓名： | 刘蕾 骆广生 吕阳成 |
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| 作者单位： | 清华大学化学工程系,北京,100084 |
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| 基金项目： | 国家自然科学基金资助项目(编号:29476243) |
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| 摘 要： | 为减小电极反应和电极距离过大对于两相电泳的影响，设计了微小型多通道两相电泳设备，并以苯丙氨酸为对象开展了双水相电泳的研究工作. 较系统地研究了时间、电流密度、料液初始浓度等因素对葡聚糖–聚乙二醇–水体系中的苯丙氨酸上下相浓度比、萃取率、总传质量以及电流效率的影响. 基于分析，给出了计算最大总传质量的半经验式.
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| 关 键 词： | 双水相 电泳 苯丙氨酸 |
| 文章编号： | 1009-606X(2003)04-0317-06 |
| 修稿时间： | 2003年3月27日 |
Influence of External Electric Field on Distribution of Phenylalanine in an Aqueous Two-phase System |
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| LIU Lei,LUO Guang-sheng,LU Yang-cheng.Influence of External Electric Field on Distribution of Phenylalanine in an Aqueous Two-phase System[J].Chinese Journal of Process Engineering,2003,3(4):317-322. |
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| Authors: | LIU Lei LUO Guang-sheng LU Yang-cheng |
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| Abstract: | In order to overcome the influences of electrode reactions and the long distance between two electrodes on two-phase electrophoresis, a mini-multi-channel aqueous two-phase electrophoresis (mini-MATPE) equipment was designed, and the separation of phenylalanine in this equipment was investigated with dextran-polyethylene glycol-water as a working system. The influences of time, current intensity, initial solute concentration on two-phase concentration ratio, recovery ratio, total mass flux, and electric current efficiency was discussed. Based on the study, a semi-empirical correlation for predicting the maximum mass flux is suggested. |
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| Keywords: | aqueous two-phase electrophoresis phenylalanine |
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