| 凝胶层析法纯化纤维素酶的研究 |
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| 引用本文： | 姚善泾,关怡新,俞丽华.凝胶层析法纯化纤维素酶的研究[J].中国化学工程学报,2002,10(6):723-725. |
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| 作者姓名： | 姚善泾 关怡新 俞丽华 |
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| 作者单位： | DepartmentofChemicalandBiochemicalEngineering,ZhejiangUniversity,Hangzhou310027,China |
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| 基金项目： | Supported by the National Natural Science Foundation of China (No. 29736180). |
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| 摘 要： | The gel filtration was carried out for purification of cellulase.The influences of chromatographic parameters on the resolution were studied to determine the optimal conditions for purification.The purified endoglucanase was obtained by gel filtration by Superdex 75 prep grade with an activity recovery of 92.8% and the purification factor 4.2.The sample volume should be below 6% of the column bed volume and the column bed height L≥12.0 cm.The optimum catalysis temperature and pH for the enzyme were 55℃and 4.5-.0 respectively.The cellulase was stable at pH ranging from 4.0 to 6.0 and temperature below 60℃.
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| 关 键 词： | 凝胶层析法 纯化 纤维素酶 表征 酶活性 |
| 修稿时间： | |
Characterization of Crude Cellulase from Trichoderma reesei and Purification of Cellulase |
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| YAO Shanjing,GUAN Yixin,YU Lihua.Characterization of Crude Cellulase from Trichoderma reesei and Purification of Cellulase[J].Chinese Journal of Chemical Engineering,2002,10(6):723-725. |
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| Authors: | YAO Shanjing GUAN Yixin YU Lihua |
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| Affiliation: | Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China |
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| Abstract: | The gel filtration was carried out for purification of cellulase. The influences of |
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| Keywords: | cellulase endoglucanase purification characterization gel chromatography |
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