大孔树脂对刺葡萄酒渣中提取的白藜芦醇的纯化工艺研究

通过静态吸附-解吸试验从6种大孔树脂中筛选出最适合刺葡萄酒渣中白藜芦醇纯化的大孔树脂，并对其进行静态、动态吸附-解吸工艺条件优化，结果表明：供试树脂中，大孔树脂H103为最适树脂，其静态吸附-解吸最优条件为：上样液质量浓度为0.65 mg/mL，上样液pH值为3，洗脱液为体积分数70%乙醇；动态吸附-解吸最优条件为：上样流速1.5 mL/min，上样液体积6 BV；洗脱流速0.5 mL/min，洗脱液体积6 BV，在此条件下，树脂H103对白藜芦醇的吸附量为55.7 mg/g，解吸率为89.86%，经树脂H103纯化后，样品纯度由11.54%提高至59.76%。

Purification process of resveratrol from Vitis davidii Foex wine residues by macroporous resin

The optimal macroporous resin for purifying resveratrol from Vitis davidii Foex wine residues was screened from the six macroporous resin with static adsorption-desorption experiments. The conditions for static and dynamic adsorption-desorption processes were optimized. Results showed that macroporous resin H103 was the optimal. The optimal static adsorption-desorption conditions were initial resveratrol concentration in the sample solution 0.65 mg/ml, pH=3, elute solution as 70% ethanol. The optimal dynamic adsorption-desorption conditions were as follows: flow rate 1.5 ml/min and volume 6 BV for adsorption, 0.5 ml/min and 6 BV for desorption. Under these conditions, the adsorption capacity of macroporous resin H103 for resveratrol was 55.7 mg/g, the desorption rate was 89.86%. The purity of resveratrol increased from 11.54% to 59.76%.