仰韶陶融型白酒大曲可培养微生物多样性研究

研究陶融型白酒大曲可培养微生物的多样性。采用组织培养方法分离陶融型白酒大曲的微生物，提取分离菌株DNA，用酵母通用引物NL1/NL4、细菌通用引物799F/1492R和真菌通用引物ITS1/ITS4对酵母26S rRNA、细菌16S rRNA、霉菌28S rRNA序列扩增，提交测序分析，构建系统发育树。仰韶陶融型白酒大曲酒曲中共分离到酵母213株、细菌386株、霉菌73株，酵母归分为72株形态种，细菌归分为77株形态种，霉菌归分为7株形态种，根据测序结果不同，陶融型白酒大曲微生物被分为21个分类单元。仰韶陶融型白酒大曲可培养微生物具有多样性特征，为其发酵及开发利用提供理论参考。

Microbial diversity of culturable microorganism from Yangshao pottery-flavor Baijiu Daqu

The diversity of culturable microorganism in Pottery-flavor Baijiu (Chinese liquor) Daqu was studied. The Daqu microorganisms in Pottery- flavor Baijiu were isolated by tissue culture method. When microbial DNA was extracted, yeast universal primers NL1/NL4 was used for 26S rRNA sequences amplification, bacteria universal primer 799F/1492R was used for 16S rRNA sequences amplification, mold universal primer ITS1/ITS4 was used for 28S rRNA sequences amplification, the sequences were analyzed and results were submitted, to construct phylogenetic tree. Results showed that there were 213 yeasts, 386 bacteria and 73 mould strains isolated from Pottery-flavor Baijiu Daqu, including 72 yeasts species, 77 bacteria species, and 7 moulds species. Based on sequencing results, the isolated strains were classified into 21 operational taxonomic units (OUTs). The culturable microorganism in Yangshao Pottery-flavor Baijiu Daqu had microbial diversity, which provided theoretical foundation for fermentation and utilization.