Specific and independent recognition of U3 and U5 att sites by human immunodeficiency virus type 1 integrase in vivo |
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Authors: | T Masuda MJ Kuroda S Harada |
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Affiliation: | Department of Biodefense and Medical Virology, Kumamoto University School of Medicine, Kumamoto, Japan. tmasu.impt@med.tmd.ac.jp |
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Abstract: | The retroviral attachment (att) sites at viral DNA ends are cis-acting regions essential for proviral integration. To investigate the sequence features of att important for human immunodeficiency virus type 1 (HIV-1) integration in vivo, we generated a series of 25 att mutants of HIV-1 by mutagenesis of the U3, U5, or both boundaries of att. Our results indicated that the terminal 11 or 12 bp of viral DNA are sufficient for specific recognition by HIV-1 integrase (IN) and suggested that IN might recognize each att site independently in vivo. |
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