Comparative Analysis of Brain Lipids in Mice,Cats, and Humans with Sandhoff Disease |
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Authors: | Rena C Baek Douglas R Martin Nancy R Cox Thomas N Seyfried |
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Affiliation: | (1) Biology Department, Boston College, Chestnut Hill, MA 02467, USA;(2) Scott-Ritchey Research Center and Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA |
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Abstract: | Sandhoff disease (SD) is a glycosphingolipid (GSL) storage disease that arises from an autosomal recessive mutation in the
gene for the β-subunit of β-Hexosaminidase A (Hexb gene), which catabolizes ganglioside GM2 within lysosomes. Accumulation of GM2 and asialo-GM2 (GA2) occurs primarily in the
CNS, leading to neurodegeneration and brain dysfunction. We analyzed the total lipids in the brains of SD mice, cats, and
humans. GM2 and GA2 were mostly undetectable in the normal mouse, cat, and human brain. The lipid abnormalities in the SD
cat brain were generally intermediate to those observed in the SD mouse and the SD human brains. GM2 comprised 38, 67, and
87% of the total brain ganglioside distribution in the SD mice, cats, and humans, respectively. The ratio of GA2–GM2 was 0.93,
0.13, and 0.27 in the SD mice, cats, and humans, respectively, suggesting that the relative storage of GA2 is greater in the
SD mouse than in the SD cat or human. Finally, the myelin-enriched lipids, cerebrosides and sulfatides, were significantly
lower in the SD brains than in the control brains. This study is the first comparative analysis of brain lipids in mice, cats,
and humans with SD and will be important for designing therapies for Sandhoff disease patients. |
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Keywords: | Sandhoff disease β -Hexosaminidase Gangliosides Glycosphingolipids GM2 GA2 Cerebrosides Sulfatides Myelin |
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