| 优化大鼠肺动脉平滑肌细胞骨架F-actin图像的激光共聚焦显微技术 |
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| 引用本文： | 郑易,施孟如,林全.优化大鼠肺动脉平滑肌细胞骨架F-actin图像的激光共聚焦显微技术[J].电子显微学报,2011,30(1):61-64. |
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| 作者姓名： | 郑易 施孟如 林全 |
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| 作者单位： | 温州医学院生物学实验教学中心;温州医学院附属第一医院呼吸科; |
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| 基金项目： | 浙江省中医药管理局资助项目(No.2010ZA084) |
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| 摘 要： | 目的:优化大鼠肺动脉平滑肌细胞(PASMCs)骨架蛋白F-actin图像的激光共聚焦显微技术.方法:采用不同浓度梯度的荧光染料及不同孵育条件,应用激光共聚焦显微扫描技术对PASMCs细胞骨架F-actin进行形态学现察.结果:终浓度10 mg/mL FITC-鬼笔环肽及10 mg/mL PI,分别染色30 min,并经...
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| 关 键 词： | 肺动脉平滑肌细胞 F-actin 激光共聚焦显微镜 FITC-鬼笔环肤 |
The optimization of the technique of laser confocal scanning microscope on the image of F-actin in rat pulmonary arterial smooth muscle cells |
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| ZHENG Yi,SHI Meng-ru,LIN Quan.The optimization of the technique of laser confocal scanning microscope on the image of F-actin in rat pulmonary arterial smooth muscle cells[J].Journal of Chinese Electron Microscopy Society,2011,30(1):61-64. |
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| Authors: | ZHENG Yi SHI Meng-ru LIN Quan |
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| Affiliation: | ZHENG Yi1,SHI Meng-ru1,LIN Quan2(1.Demonstration Center of Biological Experimental Teaching,Wenzhou Medical College,Wenzhou Zhejiang 325035,2.Department of Respiratory,The First Affiliated Hospital,Wenzhou Zhejiang 325000,China) |
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| Abstract: | Objective：To optimize the techniques of laser confocal scanning microscope（LCSM） on the image of F-actin in PASMCs.Method： Using different concentrations of fluorescent dyes and different incubation conditions,the image of F-actin in PASMCs was observed with CLSM.Results： Final concentration of 10 μg /mL FITC-Phalloidin,10 μg /mL PI,stained for 30 minutes,and worked by the Rnase at 37 ℃ were the optimal experimental conditions.The image showed that F-actin was filamentous green fluorescent light,PI labeled nuclei red fluorescence,and no significant PI staining cytoplasm.Conclusion： Through the final optimization of dyeing conditions,the experiment showed the superiority of the techniques of LCSM to observe F-actin in plane and three-dimensional cytoskeleton,which provided a good technical platform for further experiments. |
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| Keywords: | pulmonary arterial smooth muscle cell(PASMC) F-actin laser confocal scanning microscope(LCSM) FITC-Phalloidin |
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