| 进出口食品中金黄色葡萄球菌spa基因的分型 |
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| 引用本文： | 赵 芳,洪小柳,万志刚,葛丽雅,黄李华,刘慧玲,薛 峰,蒋 原,吕敬章,张 玲.进出口食品中金黄色葡萄球菌spa基因的分型[J].食品安全质量检测技术,2014,5(11):3462-3466. |
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| 作者姓名： | 赵 芳 洪小柳 万志刚 葛丽雅 黄李华 刘慧玲 薛 峰 蒋 原 吕敬章 张 玲 |
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| 作者单位： | 深圳出入境检验检疫局食品检验检疫技术中心,深圳出入境检验检疫局食品检验检疫技术中心,深圳出入境检验检疫局食品检验检疫技术中心,深圳出入境检验检疫局食品检验检疫技术中心,深圳出入境检验检疫局食品检验检疫技术中心,深圳出入境检验检疫局食品检验检疫技术中心,深圳出入境检验检疫局食品检验检疫技术中心,广东药学院食品科学学院, 广州 528458 |
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| 基金项目： | 检验检疫行业标准制修订计划项目(2013B110) |
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| 摘 要： | 目的对我国进出口食品中分离得到的金黄色葡萄球菌进行spa基因分型。方法对36株金黄色葡萄球菌的spa基因进行PCR扩增,并对产物进行测序分析,测序结果通过数据库进行spa基因分型。结果共分出16个基因型,分别为t189、t091、t164、t002、t899、t197、t1044、t034、t156、t7400、t2592、t4209、t127、t437和两株新发现的基因型。其中t189型共有13株,t091型共4株,t164和t002型分别为3株,其余型分别为1株。结论 食品中分离出的36株金黄色葡萄球菌spa分型以t189居多,t091型次之,其他14型相对较少。
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| 关 键 词： | 金黄色葡萄球菌 spa基因 多态性分析 |
| 收稿时间： | 2014/10/11 0:00:00 |
| 修稿时间： | 2014/11/19 0:00:00 |
The spa genotyping of Staphylococcus aureus isolated from import and export food |
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| ZHAO Fang,HONG Xiao-Liu,WAN ZHI-Gang,GE LI-Y,HUANG LI-Hu,LIU Hui-Ling,XUE Feng,JIANG Yuan,LV Jing-Zhang and ZHANG Ling.The spa genotyping of Staphylococcus aureus isolated from import and export food[J].Food Safety and Quality Detection Technology,2014,5(11):3462-3466. |
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| Authors: | ZHAO Fang HONG Xiao-Liu WAN ZHI-Gang GE LI-Y HUANG LI-Hu LIU Hui-Ling XUE Feng JIANG Yuan LV Jing-Zhang and ZHANG Ling |
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| Affiliation: | Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Entry-Exit Inspection and Quarantine Bureau,School of Food Science of Guangzhou pharmaceutical university, Guangzhou 528458, China |
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| Abstract: | Objective To understand of the spa genotyping of Staphylococcus aureus in import and export food. Methods The X region of the spa gene was amplified by PCR. DNA sequences were obtained with an sequencer and the spa types were determined with the database accessible via http://www. ridom.de/spaserver/. Results Thirty-six strains can be divided into 16 types, including t189, t091, t164, t002, t899, t197, t1044, t034, t156, t7400, t2592, t4209, t127, t437 and 2 new types. There are 13 strains t189 type, 4 strains t091 type, 3 strains of t164 type and 3 strains t002 type, residual type 1 strains respectively. Conclusion The main spa typing of 36 strains of Staphylococcus aureus in import and export food is t189 type, followed by t091 type, and the other 14 types less. |
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| Keywords: | Staphylococcus aureus spa gene SNP typing |
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