反相二维液相色谱法测定不同烟草制品烟草和烟气中烟碱异构体比值

为研究加热卷烟和传统卷烟烟碱异构体向主流烟气转移的差异性，建立了基于Trap柱捕获-中心切割的反相二维液相色谱法并分离分析传统卷烟和加热卷烟烟草、烟气中S-(-)-烟碱和R-(+)-烟碱异构体。采用所建立的方法研究了加拿大深度抽吸模式下，在加热卷烟和传统卷烟主流烟气中两种烟碱异构体释放量比值(R/S)的差异。结果表明:①传统卷烟烟草中的R/S约在0.16%~0.35%之间，与中心加热卷烟相当，而周向加热卷烟烟草中的R/S明显低于传统卷烟和中心加热卷烟。②传统卷烟主流烟气中的R/S明显高于加热卷烟，而中心加热卷烟主流烟气中的R/S高于周向加热卷烟；烟气中的R/S与加热卷烟和传统卷烟的使用温度有关。 

Differences of nicotine isomers'ratios in materials and mainstream smoke from different type tobacco products by reversed-phase two-dimensional liquid chromatography

In order to investigate the transfer behavior of nicotine isomers between heated tobacco products and combustible cigarettes in mainstream smoke, a reversed-phase two-dimensional liquid chromatography method based on trap column capture and heart cutting techniques was developed for separating and analyzing S-(-)-nicotine and R-(+)-nicotine isomers in cigarette smoke and heated tobacco product aerosol. The developed method was used to study the differences of R-(+)-nicotine to S-(-)-nicotine ratio (shorted as R /S ratio) in cigarette smoke and heated tobacco product aerosol under Health Canada Intense smoking regime. The results showed that: 1) The R/S ratio in tobacco of cigarette was in the range of 0.16%-0.35%, which was equivalent to that of centrally heated tobacco product; however, the R/S ratio in tobacco of circumferentially heated tobacco product was obviously lower. 2) The R/S ratio in mainstream smoke of cigarette was obviously higher than that in aerosol of heated tobacco products; that of centrally heated tobacco product was higher than that of circumferentially heated tobacco product. The R/S ratios in cigarette smoke and heated tobacco product aerosol were correlated to puffing temperature.