古田红曲米抗氧化活性物质的分离纯化

本文对产自不同地区的红曲米进行抑菌、抗氧化活性的测定,结果显示古田红曲米的抗细菌活性稍强于其它地区产的红曲米。采用凝胶柱层析、反相和正相硅胶柱层析等分离纯化方法,结合核磁共振波谱及质谱检测等技术手段对古田红曲的甲醇提取活性物质进行分析和结构鉴定。结果表明不同有机溶剂提取物的抑菌效果有很大差异,同浓度下抑菌效果从大到小依次为枯草芽孢杆菌大肠杆菌黑曲霉塔宾曲霉酿酒酵母菌;抗氧化实验中,古田红曲米抗氧化活性较强,此外,古田红曲乙酸乙酯提取物以及50%水-50%甲醇到0%水-100%甲醇提取物的抗氧化活性也较好。最后,从古田红曲米中分离纯化出2个化合物,鉴定为亚油酸和α-亚麻酸,它们均有一定的抗氧化和抗肿瘤等活性。

Separation and Purification of the Antioxidant Compounds from Gutian Red Yeast Rice

The antibacterial and antioxidant activities of red yeast rice from different areas were investigated in this paper, and the results showed that the antibacterial activity of Gutian red yeast rice was slightly stronger than that of the red yeast rice from other regions. The active compounds of Gutian red yeast rice were extracted with methanol by gel column chromatography, reversed phase and positive phase silica gel column chromatography, and then were analyzed by nuclear magnetic resonance spectrum and mass spectrometry. Results indicated that the bacteriostatic effects were different for different organic solvent extracts, and the overall order of bacteriostatic effects for extracts (under the same concentration) was as follows: bacillus subtilis >E. coli > aspergillus Niger>tower bin aspergillus >brewers yeast. In antioxidant experiment, the antioxidant activity of Gutian red yeast rice was stronger than others. In addition, the antioxidant activities of ethyl acetate extract, 50% water-50% methanol extract and 0% water-100% methanol extract of Gutian red yeast rice were also stronger. Finally, two compounds with certain antioxidant and antitumor a ctivities were identified as alpha linolenic acid and linoleic acid and were successfully separated and purified from red yeast rice.