食源性致病微生物检测技术研究进展

食源性致病微生物是引起食品安全问题的重要因素。针对食源性致病微生物，快速而准确的检测是保障食品安全的关键举措。随着微生物学、分子生物学技术的发展，基于新型基因组编辑技术和先进的生物传感器的检测技术不断涌现，食源性致病微生物的检测技术展现出多样性和综合性的特点，并得到了广泛的应用和商业化的发展。本文从食源性致病微生物分类出发，深入总结和探讨了传统培养分离法、免疫学检测技术、核酸检测技术和生物传感器检测技术的优缺点，并重点介绍了近年来开发的基于核酸等温扩增技术和CRISPR基因编辑技术的核酸检测新方法。通过对最新的针对食源性致病微生物检测方法综述，为研究者开辟食源性致病微生物检测新方法提供重要的理论参考。

Research progress of detection technologies for foodborne pathogens

Foodborne pathogens are an important cause of many food safety problems. Rapid and accurate detection of foodborne pathogens is a key measure to ensure food safety. With the development of microbiology and molecular biology technology, detection technologies based on novel genome editing technology and advanced biosensor continues to emerge . Pathogenic microorganism detection technologies have shown the characteristics of diversity and comprehensiveness, which have been widely applied and commercialized. Based on the classification of foodborne pathogens, this review thoroughly summarized and analyzed the advantages and disadvantages in allusion to culture-based methods, immunological detection technologies, nucleic acid detection technologies and biosensor detection technologies, with emphasis on the introduction of emerging nucleic acid detection methods based on nucleic acid isothermal amplification technology and clustered regularly interspaced short palindromic repeats (CRISPR) gene editing technology developed in recent years. In this paper, the latest detection methods for foodborne pathogens were reviewed, which provided important theoretical reference for researchers to develop new detection methods for foodborne pathogens.