| 假单胞菌B41产果胶酶发酵条件的研究 |
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| 引用本文： | 廖敏,陈希文,姜立春,阮期平.假单胞菌B41产果胶酶发酵条件的研究[J].中国酿造,2012(11):58-62. |
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| 作者姓名： | 廖敏 陈希文 姜立春 阮期平 |
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| 作者单位： | 绵阳师范学院分子生物学与生物制药重点实验室 |
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| 基金项目： | 科技部科技人员服务企业行动项目(2009GJF00041) |
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| 摘 要： | 对假单胞菌B41发酵产果胶酶培养基和发酵培养条件进行了优化。经单因素和正交试验得到最优发酵培养基组成为桔皮粉20．0g／L，蛋白胨10．0g／L，FeSO40．5g／L，初始pH值5．5。最佳发酵条件为装液量50mL，接种量2％，发酵温度30％，发酵时间32h。在此优化条件下，果胶酶活力为739U／mL。
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| 关 键 词： | 果胶酶 优化 发酵 |
Optimization of Pseudomonas sp. B41 fermentation conditions for producing pectinase |
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| LIAO Min,CHEN Xiwen,JIANG Lichun,RUAN Qiping.Optimization of Pseudomonas sp. B41 fermentation conditions for producing pectinase[J].China Brewing,2012(11):58-62. |
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| Authors: | LIAO Min CHEN Xiwen JIANG Lichun RUAN Qiping |
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| Affiliation: | (Key Laboratory for Molecular Biology and Biopharmaceutics,Mianyang Normal University,Mianyang 621000,China) |
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| Abstract: | The medium components and fermentation conditions for the pectinase produced by Pseudomonas sp. B41 fermentation were studied. The optimized fermentation medium determined by single factors and orthogonal test was citrus peel powder 20.0g/L, peptone 10.0g/L, FeSO4 0.5g/L, ini- tial pH value 5.5. The corresponding optimum fermentation conditions were 50ml liquid medium in a conical flask of 250ml, inoculum 2%, temperature 30% and fermentation for 32h. Pectinase activity reached 739U/ml under the optimized medium and fermentation conditions. |
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| Keywords: | pectinase optimization fermentation |
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