毛细管电泳间接激光诱导荧光法测定苦蒿和青蒿中酸性成分

利用毛细管电泳建立了中药苦蒿和青蒿及其制剂中11种酸性化合物的同时定量分析方法。其最佳电泳条件为:熔融石英毛细管50 cm(40 cm处检测窗口)×75μm i.d.;分离电压:+15 kV;分离温度:25℃;缓冲液(含:35.7 mmol/L磷酸钠,2.86×10-5mol/L荧光素钠,少量甲醇,pH=11.42);进样压力:0.5 psi(3.45 kPa),进样时间5.0 s;荧光素钠为激光诱导荧光响应试剂;LIF激发光波长:488 nm,检测波长:520 nm。该方法线性范围宽、相对标准偏差低、精密度高、重现性好,用于实际样品的测定,获得满意结果。

Determination of Acidic Constituents Conyza blinii levl.& Artemisia annua L.by Indirect LIF-CE

A novel method was established to separate and quantify simultaneously 11 acidic compounds in traditional Chinese medicine of Conyza blinii levl.and Artemisia annua L.using capillary electrophoresis(CE).Optimized apparatus conditions were given.A fused-silica capillary 50 cm(40 cm to detector)×75 μm i.d.was used.Working voltage of +15 kV was applied at 25 ℃.The buffer(pH=11.42) containing 35.7 mmol phosphate sodium,2.86×10-5mol/L fluorescein sodium(respondence reagent),and small amount of methanol was used.The hydrodynamic injection of a sample(0.5 psi(3.45 kPa)) during 5 sec was used.Indirect laser induced fluorescence(LIF) detection was applied by LIF detector at wavelength of 488 nm(excitation),520 nm(detection).The method had a wide linear range,low relative standard deviation,high precision and perfect reproducibility.Real samples assay result was achieved perfectly in this condition.