| 分光光度法测定大麦苗粉中脱镁叶绿酸的含量 |
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| 引用本文： | 王永,兰青阔,赵庆伟,赵新,朱珠,程奕.分光光度法测定大麦苗粉中脱镁叶绿酸的含量[J].食品研究与开发,2009,30(7). |
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| 作者姓名： | 王永 兰青阔 赵庆伟 赵新 朱珠 程奕 |
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| 作者单位： | 天津市农业科学院中心实验室,天津,300381 |
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| 基金项目： | 天津市科技发展计划项目 |
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| 摘 要： | 以建立测定叶绿素类产品中脱镁叶绿酸含量方法为主要目的,采用85%丙酮和乙醚萃取色素,经17%的盐酸溶液酸化后,用可见分光光度计测定667 nm下样品的吸光值,计算出脱镁叶绿酸的含量.结果表明:脱镁叶绿酸浓度在0.13mg/L～127mg/L范围内具有良好的线性关系,回归方程为Y=0.027 5X+0.002 5,R2=0.999 8.加样平均回收率为90.0%.
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| 关 键 词： | 大麦苗粉 脱镁叶绿酸 分光光度法 |
DETERMINATION OF PHEOPHORBIDE OF BARLEY GRASS POWDER BY SPECTROPHOTOMETER METHOD |
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| WANG Yong,LAN Qing-kuo,ZHAO Qing-wei,ZHAO Xin,ZHU Zhu,CHENG Yi.DETERMINATION OF PHEOPHORBIDE OF BARLEY GRASS POWDER BY SPECTROPHOTOMETER METHOD[J].Food Research and Developent,2009,30(7). |
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| Authors: | WANG Yong LAN Qing-kuo ZHAO Qing-wei ZHAO Xin ZHU Zhu CHENG Yi |
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| Abstract: | To establish a spectrophotometer determination method of pheophorbide for chlorophyll products.The pheophorbide were determined by spectrophotometry at wavelenth 667 nm. Results showed that there was a good linear relation in the range of 0.13 mg/L-127 mg/L. A standard curve was obtained and the regression equation was Y=0.027 5X+0.002 5, R2=0.999 8. The average recovery rate was 90.0%. |
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| Keywords: | barley grass powder pheophorbide spectrophotometer method |
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