高速PCR之典范SpeedCycler2

本文介绍了德国耶拿分析仪器股份公司生产的高速PCR的SpeedCycler2的性能特点和应用。重点介绍了如何通过提高PCR升降温速度,提高模块温度与样品溶液温度一致性,来极大地降低每个PCR循环的时间,从而达到提高整个PCR反应速度的目的。指出了高速PCR提高PCR产物的特异性的可能性。同时也列举了高速PCR在人类基因组学,长片段DNA植物基因组学,及在法庭科学等方面应用,指出了高速PCR技术为各项基因研究可能带来的新的进步

SpeedCycler2 with Rapid PCR Technology

The specification and application of one typical rapid PCR instruments SpeedCycler2,made by Analytik-jena.AG,Germany,are described.It is introduced that how SpeedCycler2 increased the PCR speed by increasing the PCR ramp rate,and decreasing the temperature difference between the PCR module and sample solution.The possible increasing of PCR specificity is also introduced.Some application of rapid PCR in Human Genome,long piece DNA research in Plant Genome,and forensic are listed,and the possible progress in ...