基于仿生微流控技术的肠道器官芯片构建

人体肠道具有复杂的生理环境，存在肠上皮细胞形成的绒毛结构以及流体剪切力、肠道蠕动 等力学条件，且肠道中与人体共生着大量微生物，这些与人类的健康息息相关。对于肠道的研究，传 统的体外细胞培养手段不能完全模拟肠道复杂的生理环境，具有局限性。该研究设计了一种基于微流 控技术的肠道器官芯片，通过将肠细胞培养于绒毛状基膜，结合流体剪切力来模拟人体肠道的结构与 功能。结果显示，该肠芯片重现了肠道的绒毛结构与屏障功能，提高了黏液的表达量，并实现了肠道 菌在肠芯片上的实时观察，可以成为在体外研究肠道微生物与宿主相互作用的有力工具。

Construction of Gut-on-a-Chip Based on Bio-Microfluidic Technology

The human intestinal tract has a complex physiological microenvironment, such as intestinal epithelial cells forming villi structure, fluid shear stress, intestinal peristalsis and other mechanical conditions. Moreover, a large number of microorganisms colonized in the intestinal tract which are closely related to human health. Traditional in vitro cell culture methods can not simulate the complex physiological microenvironment of the intestinal tract. In this research, we designed a microfluidic chip by culturing intestinal cells on the villous basement membrane and combining with fluid shear force to simulate the structure and function of human intestinal tract. The results showed that the intestine chip reproduced the intestinal villi structure and barrier function, increased the expression of mucus, and obtained real-time observation of intestinal bacteria on the intestine chip, which can be used as a powerful research tool for studying the interaction between intestinal microorganisms and the host in vitro.