| 黑曲霉IN7-31产糖化酶的液态发酵参数与技术优化研究 |
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| 引用本文： | 贺莹,吕利华,张婵,赵良启.黑曲霉IN7-31产糖化酶的液态发酵参数与技术优化研究[J].中国酿造,2011(1). |
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| 作者姓名： | 贺莹 吕利华 张婵 赵良启 |
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| 作者单位： | 山西大学,化学生物学与分子工程教育部重点实验室,山西大学,生物技术研究所,山西太原030006 |
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| 摘 要： | 通过N+注入、氯化锂一紫外线复合诱变方法选育了一株高产糖化酶黑曲霉IN7-31(Aspergillus niger)菌株,采用响应面法对该菌株产糖化酶的液态发酵工艺条件进行了优化。首先,通过前期的预实验,筛选出了对发酵工艺影响较大的三个培养基成分和两个培养条件,然后,分别对这两组进行了最陡爬坡实验,用最陡爬坡试验逼近最大响应区域,通过中心组合实验及响应面法分析优化了这几个因素,并进行了优化后的验证实验。利用SAS软件分析得到该菌株产糖化酶的最佳工艺条件为:玉米粉56.5g/L,豆粉22.0g/L,K2HPO4 1.3g/L,pH值为6.40,装液量80.8mL/250mL三角瓶,在此条件下摇瓶发酵黑曲霉产糖化酶的最大酶活为3044U/mL,比优化前提高了1.59倍。结果表明,通过响应面试验得到了产糖化酶黑曲霉液态发酵最佳工艺条件,可以为糖化酶的工业化生产提供一定的技术参数与条件。
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| 关 键 词： | 黑曲霉 糖化酶 液态发酵 响应面 |
Optimization of submerged fermentation parameters and technology of glucoamylase-producing Aspergillus niger IN7-31 |
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| HE Ying,L Lihua,ZHANG Chan,ZHAO Liangqi.Optimization of submerged fermentation parameters and technology of glucoamylase-producing Aspergillus niger IN7-31[J].China Brewing,2011(1). |
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| Authors: | HE Ying L Lihua ZHANG Chan ZHAO Liangqi |
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| Affiliation: | HE Ying,LU Lihua,ZHANG Chan,ZHAO Liangqi*(Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Shanxi University,Taiyuan 030006,China) |
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| Abstract: | A high glucoamylase-producing Aspergillus niger IN7-31 was obtained by mutagensis of N+ implantation and UV-LiCl.Fermentation conditions were optimized with response surface methodology(RSM).Firstly,3 ingredients of medium and 2 cultivation conditions significantly affect fermentation were selected by pre-experiment.The two groups were carried out by steepest ascent experiment,using the path of steepest ascent tests to approach the largest response region.RSM were applied to determine the optimal concentrat... |
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| Keywords: | Aspergillus niger glucoamylase submerged fermentation response surface method |
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