| 固态发酵玉米黄粉饲料复合菌种的筛选 |
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| 引用本文： | 江成英,王 松,李 琰,徐婷婷,郭宏文,张文学,韩国栋,姜未公. 固态发酵玉米黄粉饲料复合菌种的筛选[J]. 中国酿造, 2018, 37(2): 71. DOI: 10.11882/j.issn.0254-5071.2018.02.015 |
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| 作者姓名： | 江成英 王 松 李 琰 徐婷婷 郭宏文 张文学 韩国栋 姜未公 |
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| 作者单位： | 1.齐齐哈尔大学 食品与生物工程学院，黑龙江 齐齐哈尔 161006；2.齐齐哈尔大学 农产品加工黑龙江省普通高校重点实验室，黑龙江 齐齐哈尔 161006 |
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| 基金项目： | 黑龙江省教育厅基本业务专项（LTSW201708） |
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| 摘 要： | 运用平板点种法、点种刺激圈法和多菌种固体发酵法3种主要方法，筛选发酵玉米黄粉饲料的复合菌株。结果表明，枯草芽孢杆菌（Bacillussubtilis）、凝结芽孢杆菌（Bacilluscoagulans）和地衣芽孢杆菌（Bacilluslicheniformis）3种菌株以1∶1∶1的比例混合，按5% 的接种量接入发酵培养基，发酵效果较好，于30 ℃条件下发酵120 h，玉米黄粉饲料中可溶性蛋白含量由3.78%提高到14.26%。
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| 关 键 词： | 玉米黄粉 复合菌种 固体发酵 可溶性蛋白 蛋白酶活力 |
Screening of mixed strains for preparing corn gluten meal feeds by solid-state fermentation |
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| JIANG Chengying,WANG Song,LI Yan,XU Tingting,GUO Hongwen,ZHANG Wenxue,HAN Guodong,JIANG Weigong. Screening of mixed strains for preparing corn gluten meal feeds by solid-state fermentation[J]. China Brewing, 2018, 37(2): 71. DOI: 10.11882/j.issn.0254-5071.2018.02.015 |
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| Authors: | JIANG Chengying WANG Song LI Yan XU Tingting GUO Hongwen ZHANG Wenxue HAN Guodong JIANG Weigong |
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| Affiliation: | 1.College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, China; 2.Key Laboratory of Processing Agricultural Products of Heilongjiang Province, Qiqihar University, Qiqihar 161006, China |
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| Abstract: | The mixed strains for fermented corn gluten meal feed were screened by dot-inoculating, dot-inoculating stimulus circle method and multi-strains solid-state fermentation. The results showed that the three strains of Bacillussubtilis, BacilluscoagulansandBacilluslicheniformiswere mixed by 1∶1∶1 with inoculum 5% in the fermentation medium, the fermentation effect was better. Under the conditions of 30 ℃ for fermentation 120 h, the soluble protein content in corn gluten meal feed increased from 3.78% to 14.26%. |
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| Keywords: | corn gluten meal mixed strains solid-state fermentation soluble protein protease activity |
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