响应面法优化固相微萃取生板栗香气成分条件

利用响应面法研究不同萃取参数对生板栗香气成分萃取效果的影响。分别优化固相微萃取的萃取纤维头、萃取温度、平衡时间、萃取时间等因素，测定其对生板栗香气成分的种类、总峰面积的影响。结果表明，最佳萃取条件为65 μm聚二甲基硅烷/二乙烯苯（PDMS/DVB）萃取头、平衡时间18 min、萃取时间50 min、萃取温度58.5 ℃，在此优化条件下经气质联用法检测出生板栗香气成分21种，总峰面积48 382.7。其中醛类占14.40%、醇类占7.06%、酮类占7.72%、酯类占52.97%。

Optimization and analysis of the SPME extraction parameters of raw chestnut aroma components by response surface methodology

The effects of different extraction parameters on the extraction of raw chestnut aroma components were studied by response surface method. The solid-phase microextraction (SPME) extraction fiber head, temperature, balance time and extraction time were optimized, and the effects of extraction factors on types of raw chestnut aroma components and total peak area were determined. Results showed that the optimum extraction conditions were 65 μm fiber head with poly dimethyl silane/divinyl benzene (PDMS/DVB), balance time 18 min, extraction time 50 min and temperature 58.5 ℃. Under the conditions, 21 kinds of aroma compounds of raw chestnut were detected, and the total peak area was 48 382.7 by GC-MS. Aldehydes, alcohols, ketones and esters in the aroma compounds accounted for 14.40%, 7.06%, 7.72% and 52.97%, respectively.