直接法制备188Re-碘化油

探讨直接法制备188Re-碘化油的实验条件及标记物性质.在温度为75～120℃和不同实验条件下,直接标记法制备188>Re-碘化油,观测标记物标记率在室温和37℃人血浆体系中随时间的变化情况;采用红外光谱仪分析标记对碘化油分子结构的影响;用肿瘤模型动物显像验证碘油对188Re的包埋效果,以及在生物体内的稳定性半定量分析.直接法制备188Re-碘化油,在最佳标记条件下,标记物标记率可达(99.1±0.4)%;标记物在室温和37℃人血浆体系中放置3 d,标记率可保持在(90.9±1.9)%和(92.8±1.2)%.红外光谱结果显示本标记方法不改变碘化油的分子结构;荷S180骨肉瘤鼠显像表明,瘤内注射标记物48 h后,肿瘤部位仍有明显的放射性浓聚.188Re直接标记碘化油易于操作,标记物标记率高,体外稳定性好,碘油对放射性核素铼的包裹效果良好.

Preparation of ~(188)Re-Lipiodol With Directly Radiolabeling Method

The aim of this study is to explore the method of radiolabeling lipiodol with ¹⁸⁸Re and to appraise the quality of the product. Lipiodol was labeled with ¹⁸⁸Re directly at the temperatures from 75 ℃ to 120 ℃ with different reaction conditions. The time-variation labeling efficiency was measured to evaluate the stability of ¹⁸⁸Re-lipiodol.The molecular structure change caused by radiolabeling was analyzed with infrared spectrometer. The embedding effect of lipiodol was explored by SPECT imaging of bearing cancer S180 mice. The optimal condition was obtained with the labeling efficiency over (99.1±0.4) %. More than (90.9±1.9)% and (92.8±1.2)% of radioactivity was kept in lipiodol form in room temperature and in 37 ℃ human plasma for 3 d. Infrared spectrum imaging shows that the molecular structure of radiolabeled lipiodol is unchanged. SPECT imaging of S180 tumor bearing mice testifies the radioactivity concentrated in tumor for 48 h after injection of ¹⁸⁸Re-lipiodol. The directly radiolabeling method is easy to operate, and the labeling efficiency is higher than (99.1±0.4)%. The radiolabeled product is stable for 72 h, and the embedding effect of lipiodol to ¹⁸⁸Re is well. ¹⁸⁸Re radiolabeled lipiodol can be used for further investigation.