不同波长光照射血液诱发的荧光光谱研究

为研究波长因素在激光与血液相互作用中的影响 ,从健康人静脉采集血液 ,分别用 5 0 2nm ,5 30nm和 6 32 8nm波长光照射血液样品 ,同时用光栅光谱仪检测其荧光光谱。结果表明 ,5 30nm波长光在血液中引发的荧光辐射最强 ;5 0 2nm波长光在血液中引发的荧光辐射比较弱 ;6 32 8nm波长光照射血液 ,所产生的发射光谱在原激发光的长波和短波两个方向都有分布。这一结果提示 ,上述三种波长光与血液相互作用的过程有所不同 ,因而其对血液的生物效应也会有所差异。

Study of Fluorescent Spectrum of Blood Induced by Lights of Different Wavelengths

The blood taken from a healthy human vein is irradiated by different wavelength lights at 502 nm, 530 nm and 632.8 nm. The fluorescent spectra are tested by the grating spectrograph. The result indicates that the fluorescent intensity of the blood induced by the light at 530 nm is the strongest. The fluorescence of the blood induced by the light at 502 nm is very weak. The emitting spectrum of the blood induced by the light of 632.8 nm appears in both side of 632.8 nm. This result shows that the processes of interaction between laser and blood may be different when wavelengths of the exciting lights are different. So the biological effects of the lights on the blood may be different.