光学蛋白质芯片技术及其应用

本文介绍一种新型光学蛋白质芯片技术和在生物医学领域的应用。通过固体基底表面的微格式化，表面改性和生物配基固定等技术，制备多元生物活性感应表面；利用生物分子操作，蛋白质的特异结合性和高分辨率的生物光学椭偏显策成像技术，达到识别，检测和纯化蛋白的目的，它是一种非标记的多元蛋白质定量分析方法，并且能够对蛋白质之间的相互作用过程进行实时测量，获得反应速率及反应条件等生物分子反应的动力学参数。

Optical Protein Chip and Its Applications

One kind of optical ProteinChip, and a ProteinChip analysis system, as well as some applications are introduced in this paper. The chip is prepared with surface patterning, surface modification, ligand immobilization to form a sensing surface with multi-bioactivity. The affinity between proteins, and the optical imaging ellipsometry with high spatial resolution are used to realize non-labeling micro-assays for protein identifications and protein interactions. It is not only a qualitative, but also a quantitative way for protein interaction determination. It is able to obtain dynamic data of protein interaction process, such as interaction rates and conditions, with real-time detection.