| 木瓜凝乳蛋白酶的分离纯化 |
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| 引用本文： | 邓静,丁玉兰,赵树进.木瓜凝乳蛋白酶的分离纯化[J].化工中间体,2005(1):34-37. |
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| 作者姓名： | 邓静 丁玉兰 赵树进 |
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| 作者单位： | 1. 四川理工学院生物工程系
2. 广州军区陆军总医院药学部 |
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| 摘 要： | 采用硫酸铵分级沉淀与离子交换相结合的方法从市售的木瓜蛋白酶干粉中分离纯化木瓜凝乳蛋白酶。结果表明Macro-Prep High S Support离子交换树脂比SP-Sepharose Fast Flow离子交换树脂能更好的分离纯化木瓜凝乳蛋白酶，经Macro-Prep High S Support交换树脂二次纯化的木瓜凝乳蛋白酶已达到了与标准品同样的纯度。
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| 关 键 词： | 木瓜凝乳蛋白酶 分离纯化 市售 标准品 SP 离子交换树脂 干粉 硫酸铵分级沉淀 纯度 相结合 |
| 文章编号： | 1672-8114(2005)01-0034-04 |
The purification of chymopapain |
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| Authors: | Deng Jing Ding Yulan ZHAO Shujin |
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| Affiliation: | Deng Jing1 Ding Yulan2 Zhao Shujin2 |
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| Abstract: | Chymopapain was purified from commercially available spray-dried latex of papaya fruit by (NH4)2SO4 fractionation and protein chromatography on SP-Sepharose Fast Flow and Macro-Prep High S Support cation-exchange column respectively. The results indicated Macro-Prep High S Support was superior to SP-Sepharose Fast Flow resin. The chymopapain whose purity equal to standard substance was get by twice protein chromatography on Macro-Prep High S Support. |
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| Keywords: | chymopapain (NH4)2SO4fractionation ion exchange chromatography purification |
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