|
|||||
|
|
| 生淀粉酶产生菌的筛选和研究 | |
| 引用本文： | 夏媛媛,赵华,董晓宇,牛堃,杨晶. 生淀粉酶产生菌的筛选和研究[J]. 中国酿造, 2012, 31(1): 154-158 |
| 作者姓名： | 夏媛媛 赵华 董晓宇 牛堃 杨晶 |
| 作者单位： | 天津科技大学生物工程学院,工业微生物教育部重点实验室,天津市工业微生物重点实验室300457 |
| 摘 要： | 利用透明圈法再经单菌分离纯化从土壤中筛到一株产酶为158U/mL的生淀粉酶产生菌TCCC451031,经16S rDNA鉴定为Paenibacillus属。最适发酵培养基:碳源为2%的玉米粉,最佳氮源为酵母粉与玉米浆混用比例为1∶2,用量为1%。最适发酵条件:35℃、pH值为7.0、转速160r/min,发酵5d酶活达312U/mL。该酶最适作用温度40℃,最适作用pH值为4.2,Ca2+、Mg2+、Zn2+对酶有一定的激活作用,Cu2+、Mn2+、K+、Fe2+、Ba2+对酶活有抑制作用。 |
| 关 键 词： | 生淀粉酶 发酵条件 酶学性质 |
Isolation of amylase-producing strain and its enzymatic properties |
|
| XIA Yuanyuan , ZHAO Hua , DONG Xiaoyu , NIU Kun , YANG Jing. Isolation of amylase-producing strain and its enzymatic properties[J]. China Brewing, 2012, 31(1): 154-158 | |
| Authors: | XIA Yuanyuan ZHAO Hua DONG Xiaoyu NIU Kun YANG Jing |
| Affiliation: | (Bioengineering College of Tianjin Science ＆ Technology University,Institute of Biotechnology,Ministry of Education Key Laboratory of Industrial Microbiology,Tianjin Key Laboratory of Industrial Microbiology,Tianjin 300457,China) |
| Abstract: | TCCC451031 producing amylase was isolated from soil with the methods of transparent circles and single separation and purification.The amylase activity of the strain was 158U/ml.The strain was identified as Paenibacillus sp.by amplification,sequencing and alignment analysis of 16S rDNA sequence.The optimal fermentation conditions were as followed： carbon source 2% cornmeal,nitrogen sources 1% corn steep liquor and yeast powder,the ratio of corn steep liquor and yeast powder 1∶ 2,temperature 35℃,pH value 7.0 and rotation speed of shaking flask 160r/min.Under these conditions,the enzyme activity reached 312U/ml after 5d.The optimum catalysis temperature and pH value were 40℃ and 4.2,respectively.The enzyme activity was stimulated by Ca2＋,Mg2＋ and Zn2＋,while was inhibited by Cu2＋,Mn2＋,K＋,Fe2＋ and Ba2＋. |
| Keywords: | raw amylase fermentation condition enzyme property |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |