| 荧光法测定枸杞子中总黄酮含量 |
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| 引用本文： | 冯俊霞,陆敏,戚秀菊.荧光法测定枸杞子中总黄酮含量[J].中国酿造,2012,31(4):168-170. |
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| 作者姓名： | 冯俊霞 陆敏 戚秀菊 |
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| 作者单位： | 1. 石家庄学院化工学院,河北石家庄,050035
2. 石家庄学院国有资产管理处,河北石家庄,050035 |
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| 基金项目： | 河北省教育厅科学研究计划项目 |
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| 摘 要： | 该文建立了枸杞子中总黄酮含量测定的新方法。以槲皮素为标准品用荧光分光光度法测定,该方法的检出限为2.51×10-7g/L,建立的线性回归方程为y=531.84048x-3.54448,相关系数R2=0.99903,线性范围在6.7×10-6g/L～6×10-4g/L之间;枸杞子中总黄酮的平均含量1.7098μg/g,样品测定的RSD为1.6376%,平均回收率95.75%。结果表明,该方法操作简便,准确度高,可以用于枸杞子中总黄酮含量测定。
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| 关 键 词： | 枸杞子 总黄酮 荧光分光光度法 |
Determination of total flavonoids in Lycium barbarum L.by spectrofluorimetry |
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| FENG Junxia
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LU Min
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QI Xiuju.Determination of total flavonoids in Lycium barbarum L.by spectrofluorimetry[J].China Brewing,2012,31(4):168-170. |
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| Authors: | FENG Junxia LU Min QI Xiuju |
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| Affiliation: | 1.School of Chemical Engineering,Shijiazhuang University,Shijiazhuang 050035,China;2.Adminstative Office of State Owned Assets,Shijiazhuang University,Shijiazhuang 050035,China) |
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| Abstract: | Quercetin was used as standard to establish a new spectrofluormetric method for determination of total flavonoids in Lycium barbarum L.The detection limit was 2.51×10-7g/L,the range of linearity with concentration of quercetin was between 6.7×10-6g/L～6×10-4g/L,linearity relationship was y=531.84048x-3.54448,correlativity R2 = 0.99903.The average amount of total flavonoids in Lycium barbarum L.was 1.7098μg/g,RSD 1.6376%,and the average recovery was 95.75%.The method is simple and accurate for determination total flavonoids in Lycium barbarum L. |
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| Keywords: | Lycium barbarum L total flavonoids spectrofluorimetry |
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