Analysis of antibiotic resistance patterns and detection of mecA gene in Staphylococcus aureus isolated from packaged hamburger |
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| Authors: | Shahraz Farzaneh Dadkhah Hasan Khaksar Ramin Mahmoudzadeh Maryam Hosseini Hedayat Kamran Manijeh Bourke Paula |
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| Affiliation: | a Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Science and Food Technology, Shaheed Beheshti University of Medical Sciences, Tehran, Iranb Food and Health Research Centre, School of Food Science and Environmental Health, Dublin Institute of Technology, Dublin, Ireland |
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| Abstract: | Presence of Staphylococcus aureus, antibiotic resistance pattern and PCR detection of mecA gene in isolated strains were investigated in total of 256 packaged hamburgers in Iran-Tehran. For this purpose we used standard disk-diffusion method and sensitive and specific PCR technique, respectively. Results showed that 25% of samples were positive for S. aureus. Resistance to meticillin, erythromycin, penicillin G, cefazolin, ciprofloxasin, vacomycin and amoxiclave was determined 89%, 20.3%, 18.7%, 15.6%, 14%, 26.6% and 12.5%, respectively. According to the obtained results from PCR analysis of methicillin-resistant S. aureus (MRSA), mecA gene was present in 100% of the resistant isolates, 0% of intermediate-resistance isolates and 25% of susceptible isolates. The results obtained from PCR detection of mecA gene showed high correlation with standard disk diffusion test. |
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| Keywords: | MRSA Antibiotic resistance Staphylococcus aureus Hamburger mecA gene |
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