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| 响应面分析应用于短杆菌产酶工艺的优化 | |
| 引用本文： | 杨胜利,张慧,石轩峰,李亚琴,王武.响应面分析应用于短杆菌产酶工艺的优化[J].中国粮油学报,2004,19(6):60-62. |
| 作者姓名： | 杨胜利 张慧 石轩峰 李亚琴 王武 |
| 作者单位： | 1. 浙江工业大学药学院,浙江杭州,310014 2. 江南大学食品学院,江苏无锡,214036 3. 江南大学工业生物技术教育部重点实验室,江苏无锡,214036 |
| 摘 要： | 本文研究了用响应面分析法(RSM)优化短杆菌产胆固醇氧化酶的工艺，发现胆固醇添加量及超声处理时间是影响短杆菌产酶最重要的因素。在模拟优化的条件下：胆固醇为4．076g／L，吐温-80为0．2932％(v／v)，照射时间为22．361min，COD最大产量1．483U／mL。 |
| 关 键 词： | 胆固醇 吐温-80 照射时间 超声处理 影响 因素 发现 短杆菌 响应面分析 添加量 |
Optimization of Technology by Response Surface Methodology:Producing Cholesterol Oxidase From Brevibacterium sp. |
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| Yang Shengli Zhang Hai Li Yaqin Wang Wu.Optimization of Technology by Response Surface Methodology:Producing Cholesterol Oxidase From Brevibacterium sp.[J].Journal of the Chinese Cereals and Oils Association,2004,19(6):60-62. | |
| Authors: | Yang Shengli Zhang Hai Li Yaqin Wang Wu |
| Abstract: | An efficient process for producing cholesterol oxidase by Brevibacterium sp. was developed. The process was optimized by analysis of parameters including cholesterol concentration, tween-80 concentration and treatment time, and by employing Response Surface Methodology (RSM). The obtained optimum operational conditions are:cholesterol concentration 4. 076g/L, culture medium tween - 800. 2932% (v/v) , and irradiation time 22. 361 min. COD production is up to 1.483 U/ml. |
| Keywords: | cholesterol oxidase ultrasonic response surface methodology Brevibacterium sp |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |