| 响应面法对酶解蛋膜角蛋白的优化研究 |
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| 引用本文： | 张娴,高新,杨德玉,常春然,崔易.响应面法对酶解蛋膜角蛋白的优化研究[J].食品科学,2006,27(10):396-399. |
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| 作者姓名： | 张娴 高新 杨德玉 常春然 崔易 |
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| 作者单位： | 西北大学化工学院； 西北大学化学系 |
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| 摘 要： | 研究并分析了蛋膜中角蛋白的提取方法。在对反应时间、温度、酶用量、pH等单因素实验基础上,采用Box-Benhnken响应面分析法系统探讨了蛋膜中角蛋白最优的提取条件。结果表明,在温度64℃、反应时间2.2h、酶用量4.6%和pH9.3时,角蛋白产率可达38.0%,角蛋白的含量可达76.87%。
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| 关 键 词： | 蛋膜 酶解 提取工艺条件 响应面分析法 |
| 文章编号： | 1002-6630（2006）10-0396-04 |
| 收稿时间： | 2006-07-09 |
| 修稿时间： | 2006-07-09 |
Optimization of the Enzymatic Hydrolysis of Keratin via Response Surface Method |
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| ZHANG Xian,GAO Xin,YANG De-yu,CHANG Chun-ran,CUI Yi.Optimization of the Enzymatic Hydrolysis of Keratin via Response Surface Method[J].Food Science,2006,27(10):396-399. |
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| Authors: | ZHANG Xian GAO Xin YANG De-yu CHANG Chun-ran CUI Yi |
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| Affiliation: | 1.Department of Chemical Engineering,Northwest University,Xia’n 710069, China; 2.Department of Chemistry,Northwest University, Xia’n 710069, China |
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| Abstract: | To study and analyze the extraction of keratin from egg-shell membrane. On the base of the single factor experiments and the Box-Benhnken design, the desired conditions of extraction of keratin are concluded. They are 64℃ of the temperature, 4.6% of the enzyme dosage, 2.2 hours of the total reaction time, 9.3of pH. Our present results showing that the yield of keratin was 38.0% and the content of keratin was 76.87% under the above-mentioned conditions. |
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| Keywords: | egg-shell membrane enzymatic hydrolysis extracting conditions response surface method(RSM) |
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